RESULTS AFTER APPLICATION OF THE EXTRACELLULARBOVINE-DERIVED PERITONEUM MATRIX FOR ABDOMINALWALL RECONSTRUCTION IN THE LONG-TERM EXPERIMENT

Abstract

Introduction. Despite the sufficient number of works on the problem of the use of xenogeneic
implants in hernioplasty in the world science, it is necessary to recognize the ambiguity of the data
presented by different scientists, and the absence in these studies of the use of extracellular xenograft
matrix in abdominal wall reconstruction determines the relevance of this study.
The aim was to study the structural changes in the implantation area of the extracellular bovinederived peritoneum matrix with the tissues of the anterior abdominal wall in the long term of the
experiment.
Materials and methods. In the period of 2016-2017yy a comparative experimental study was
carried out to study the long-term results of the application of a new biological implant, the extracellular
bovine-derived peritoneum matrix, for abdominal wall reconstruction on 16 white nonlinear short-haired
rats of both sexes, weighing 180-220g on the basis of the vivarium of the Karaganda State Medical
University.
Using the methods of descriptive morphology and morphometry, a characteristic of the wound
process after test material implantation is given. Statistical analysis of the obtained data was carried out
by calculating the group mean ( ), standard deviation (SD). The reliability of the differences between
the groups studied was determined using nonparametric criteria: the Mann-Whitney criterion for
comparing independent groups ("experience-control"), the X2 criterion for estimating the differences
between the shares in the groups.
Results. The specific features of the course of the wound process in the long-term experiment after
the application of the new material were studied. Morphologically it was determined that by the 180th
day of the postoperative period a consistent connective tissue scar was observed without any
inflammation signs. The quantitative profile of the cellular infiltrate to 180 days after implantation,
statistically significantly changes towards increasing the number of structural stromal cells with an
inversely proportional decrease in the cells of the exudative and proliferative phases of the
inflammatory-regenerative process in comparison with the initial observation periods.
Conclusion. Based on the results of the experiment, the new biological implant at this stage
demonstrates an adequate biocompatibility with organism, with the formation of a mature, consistent
contact with tissues of the anterior abdominal wall without any inflammatory responses.