METABOLIC EFFECTS OF COMBINED INTEGRATION OFADENOSINE AND ADENOSINE MONOPHOSPHAT INHYPERADENALEMEMIA

Abstract

Introduction. It is known that adenosine as a metabolite of adenosune monophosphate (AMP) has a
variety of functional and metabolic effects aimed at maintaining homeostasis. Adenosine plays a
particularly important role in the adaptation of the myocardium to ischemia and hypoxia. By exerting an
antiarrhythmic effect on the heart, adenosine slows the atrioventricular conduction induced by
catecholamines.
Purpose of the study. To study the effects of the combined effect of the adenosine + AMP complex
on the immune status, on the activity of antioxidant protection enzymes and purine nucleotide
metabolism, on the level of MDA and DC in the blood, in the heart and liver in hyperadrenalinemia.
Material and methods. Study design: experimental. In the blood, in the heart and liver of white rats
against the background of hyperadrenalinemia, created in / b adrenaline injection at a dose of 0.4 mg
per 100 g of body weight 60 minutes before the study, the effect of the adenosine + AMP complex at a
total dose of 1000 μg on the immune status, On the activity of glutathione peroxidase (GРO),
glutathione reductase (GR), catalase, adenosine deaminase (AD), AMP-deaminase (AMPD), 5'-
nucleotidase (5'H), on the level of malonic dialdehyde (MDA) and diene conjugates (DC).
The results of the study were processed using the t-test of the Student. The paper presents the
arithmetic mean ± mean error (X ± m). Differences were considered significant at p <0.05.
Results. In hyperadrenalemia, the introduction of the adenosine and AMP complex reduces the total
number of leukocytes, T-lymphocytes and T-suppressors, increases T-lymphocytes and RTML,
increases the activity of the enzymes of metabolism of purines AD, AMPD, 5'H and GR, reduces the
activity of GPO, catalase and the level MDA in the blood
In the heart with sympathetic hyperactivation, the complex of adenosine and AMP leads to the
activation of AD and AMPD, to an increase in the ratio of the activities of AD + AMPD / 5'H towards the
catabolism of adenosine and AMP, causes a decrease in the amount of MDA and DC, and the activity
of GR, GPO and catalase, which indicates a decrease in peroxidation processes in this organ.
In the liver with hyperadrenalinemia, the adenosine and AMP complex reduces the level of MDA and
DC, and the activity of GAP and catalase decreases accordingly, thereby reducing the level of oxidative
stress caused by adrenaline.
Conclusions. To correct the changes in the antioxidant defense system, the activity of the purine
metabolism enzymes and immune responses observed in hyperadrenalemia and oxidative stress of any
origin, a complex of adenosine and AMP can be used.