EFFECT OF EXTRACT ON ACTIVITY OF ENERGYMETABOLISM ENZYMES AFTER COMBINED EFFECTOF EMOTIONAL STRESS AND SUBLETHAL DOSEOF γ-RADIATION IN REMOTE PERIOD

Abstract

Introduction. During adaptation process complex changes occur in metabolic pathways; functional
and structural changes occur in organs and tissue. Despite the great interest of researchers to this
problem, the pathogenetic mechanisms of influence of stress, especially combined effect of stressors on
the metabolic processes hasn’t been studied enough. Main criterion for the stability of the adaptive
behavior of the organism is to maintain energy potential of biosystems constant. Given the importance
of this system, it is necessary to define its role in formation of pathological process in animals in remote
period under combined effect of γ-radiation and emotional stress and their corrections.
Purpose – study effect of extract Eminium Regelii on activity of energy metabolism enzymes in
immunocompetent organs in remote period under combined effects of sublethal doses of γ-radiation (6
Gy), and emotional stress.
Materials and methods. To achieve this goal, experiments were performed on 70 white male
laboratory Wistar rats, weighing 240 ± 20 g, which were divided into 7 groups. Emotional stress was
modeled in animals by tail suspension, and were withdrawn from the experiment after 1, 2, 3 days by
partial decapitation, previously put to sleep with ether anesthetics. Prior to research, animals were
exposed to radiation once with 6 Gy for 90 days on radiotherapy installation TERAGAM Сo
60
(«ISOTREND spol. s.r.o.», Czeck Republic)
Results. Decrease in activity of succinate dehydrogenase in spleen, adrenal glands and peripheral
blood lymphocytes and in all investigated organs cytochrome oxidase’s activity has shown stressed
conditions of metabolic and enzymatic processes, leading to a shortage of energy supply. Under
combined effects, result was more pronounced on day 3. Research has shown positive effect of the
extract in increasing enzyme activity.
Conclusion. Under effect of double factors – combination of sublethal doses of γ-radiation and
emotional stress in remote period, a decline in the activity of energy metabolism enzymes occur.
Administration of 2.5 ml / kg of Eminium Regelii causes an increase in enzyme activity, which was
decreased as a result of combined effects.