ESTIMATION OF OXIDATIVE STRESS VIOLATIONAT EXPERIMENTAL NEPHROPEXY

Abstract

Аctuality. The choice of implant, always found attention in the views of scientists and surgeons.
Synthetic materials have undergone significant changes as new technologies are discovered. But today,
their use causes a lot of heated discussions, disputes and various responses. Therefore, the further use
of implants is one of the promising and relevant areas of surgery.
Objective: to study the application effect of polyester mesh, macroporous partially absorbable light
mesh UltraPro and own peritoneal-fascial flap in the simulation of nephropexy in the experiment on the
processes of free radical oxidation, endogenous intoxication and antioxidant protection.
Materials and methods. Experimental comparative research was conducted on the basis of
laboratory of Department of molecular biology of Karaganda state medical University in the period 2016-
2017.
The experiment was carried out on 144 white mature free rats of both sexes, which were divided into
three groups of 48 rats, depending on the method of fixing the kidney. Terms necropsy animals from the
experiment amounted to 7, 14, 21, 30, 90 and 180 days.
For the evaluation of free-radical oxidation was used to determine secondary products of lipid
peroxidation: malondialdehyde and antioxidant enzymes - glutathione peroxidase. Was used to
determine the medium-molecular peptides for the assessment of endogenous intoxication. The sample
in subgroups of 8 animals was sufficient to identify differences in frequency at 5% confidence level and
95% confidence level. This calculation of the sample and the population is made by statistical analysis
using the package «Statistica 8.0 StatSoft Inc. 2008».
Results. Indicators of oxidative stress showed that the content of Malone dialdehyde has a wavelike
nature, but with a tendency to decrease. Inhibition of glutathione peroxidase on the 14th, 21st, 30th day
in all types of implants was revealed. The results showed that against the background of increased
activity of the enzyme glutathione peroxidase, the preservation of endotoxication is noted, as evidenced
by the increased content of medium-molecular peptides on the 180th day.
Conclusion. The results expand the understanding of the influence of the implant, justifying the
possible reactive changes in parenchymal and connective tissue components of the kidney. The results
substantiate the possibility of clinical trials for the study of UltraPro light mesh.