MORPHOLOGICAL CHARACTERISTICS OF GRANULOSACELLS OF MICE OVARIAN UNDER LINDANE EXPOSURE

Abstract

Introduction. Analysis of materials that characterize the human environment in the Aral Sea, has
shown a sharp increase of the chemical (pesticides, fertilizers, salt components, heavy metals) pollution
of drinking water, air, soil, pesticide contamination of food products of plant and animal origin, fishery
products.
Aim. Еvaluate the ultrastructural changes in granulosa cells of mice under the exposure of lindane
pesticide in vitro.
Methods. The experimental work carried out in the Laboratory Centre of Microscopy Department of
Life, Health and Environmental Sciences University L’Aquila, Italy; 80 female mice control (10), control
with solution of dimethyl sulfoxide (10), lindane 1 μM (20), lindane 10 μM (20), lindane 100 μM (20). The
material of the study were ovarian granulosa cells. After preparing the samples for transmission electron
microscopy, ultra-thin sections (60-80 nm) were obtained on an ultramicrotome and contrasted with
salts of heavy metals with uranyl acetate and lead citrate (Sic Rome, Italy).
Results. Comparative analysis of the group showed the dependence of ultrastructural changes in
the applied lindane concentration.The presence of irregularly shaped cells were observed early signs of
fragmentation of the cytoplasm, which was filled with a plurality of mitochondria and fatty droplets. In the
group with the highest concentration of toxicant cells appeared marginalization of chromatin remnants of
apoptotic bodies and single organelles.
Conclusion. The exposure of lindane in vitro have shown a dose-dependent toxicity effect on the
granulosa cells; ultrastructural changes caused granulosa cells strictly correlated on the applied dose.